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| Assistant Professor |
| Office:
919-966-6915 |
| E-mail:
seigo_hatada@med.unc.edu |
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My research interest is to apply gene targeting for curing genetic diseases. The current methods of gene therapy have problems caused by random insertion of viral sequences into the genome. I would like to develop a safer method for gene therapy based on gene targeting.
I have already demonstrated the feasibility of gene targeting in mouse hematopoietic progenitor cells (Hatada et al., 2000). It is in principle possible to correct a faulty gene by gene targeting in most stem/progenitor cells. But we have no good method for purifying the gene-corrected cells from un-corrected cells. I have recently developed a promising method for enriching gene-corrected mouse embryonic stem cell by introducing fluorescence genes into a gene-correcting vector (Hatada et al., 2005). The method can separate gene-corrected cells quickly by using FACS. I hope to apply this method to correct faulty genes in other types of stem cells including those from humans. |
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| Isolating gene-corrected stem cells without drug selection. Hatada S, Arnold LW, Hatada T, Cowhig J Jr, Ciavatta D, Smithies O Proc Natl Acad Sci U S A. 2005; 102:16357-61. |
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| Correction of factor IX deficiency in mice by embryonic stem cells differentiated in vitro. Fair JH, Cairns BA, Lapaglia MA, Caballero M, Pleasant WA, Hatada S, Kim HS, Gui T, Pevny L, Meyer AA, Stafford DW, Smithies O, Frelinger JA. Proc Natl Acad Sci U S A. 2005; 102:2958-63. |
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| Gene correction in hematopoietic progenitor cells by homologous recombination. Hatada S, Nikkuni K, Bentley SA, Kirby S, Smithies O Proc Natl Acad Sci U S A. 2000; 97:13807-11. |
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| The influence of chromosomal location on the expression of two transgenes in mice. Hatada S, Kuziel W, Smithies O, Maeda N J Biol Chem. 1999; 274:948-55. |
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